Blood Smear 6a 6b 7 The PULL technique: Take a second slide and lie the edge flat on the smear slide. 22 Jan 2021 by Goblusal. Use clean, high-quality microscope slides. Preparation of blood smear 6. Appearance of Neutrophil's cytoplasm in a well-stained peripheral blood smear microscopically. <>
Smear technique. Materials 2 (75mm x 25mm) clean glass slides. 2) Use the second slide as a “spreader” slide. The three main blood cells that the test focuses on are: red cells, which carry oxygen throughout your body 10 0 obj
Slide three? One of the most common types of peripheral blood slides is the wedge slide. %PDF-1.5
The technique for making and storing dried blood samples is given in the section “Dried Blood Samples”. Second slide (ideally narrower than smear slide, to avoid spreading the cells over the edge) is placed on the smear slide in front of the blood spot in a … Knowing the patient’s age and health status can help you determine the best way to make your slide. Peripheral Blood Smear. In infants, puncture the heel. Tossed. This site uses Akismet to reduce spam. endobj
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Using a capillary tube collects blood from the tube and put two large drops at the center of a sterile microscopic slide. Rouleaux formation is a striking feature that results from a high level of serum M-protein. endobj
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When I make a slide, I know what adjustments to make as soon as I see the blood spread against the pusher slide. endobj
I hold the patient slide in my other hand the same way as the pusher slide but held perpendicular to it to give me more stability. Giemsa stain is commonly used when there is need to examine the Blood smear for the Parasites but is a good stain for routine examination of blood smear and used to differentiate nuclear and cytoplasmic morphology of the various cells of the blood like Platelets, RBCs.... Giemsa Staining Technique… A blood smear involves looking at a sample of blood under the microscope after applying special stains and looking for abnormalities or changes in red blood cells, white blood cells, and platelets. 6. There are many reasons why your doctor may order a blood smear. Types of thin film technique. However, the effectiveness and accuracy of that interpretation depend on the quality of the slide. stream
4. Place a drop of blood on the patient slide near the frosted edge. 11 0 obj
Thinner blood behaves exactly the opposite as blood from patients with high hematocrit levels. 5. endobj
Wipe away the first drop of blood with clean gauze. In the end, all that matters is that the pusher slide edge is flat against the patient slide. Hold the spreader slide at 30-40 degrees to achieve optimal smear length. x��V�n�0��?̑`��H r��"A�.q�CЃ"3�RYJe'F��C-N���bs��7��c�����.ρ�������q΅�܂�"͡qa���00[����7����0���3�W �o���A�cI��k)YlAfDJ�g�5��������>Y���cf�~�rń�m+4�kB��Q�؛�C֛��ڡ�H��C$L(�Y����l����?.�U:�����Ӛl�u��o��w����+�.�%$\�� ������1��CZ̀�j����#�t�wT��{?cp�`�)�Ak'���7�Ƣp��᳙��~���%�\����TD�-�w�\���d7����os��q����K��2�,x�ro�-�c�mr�B��
Ù�J ��@�fp�� Preparation of thin blood smear. The other is pusher or the spreader slide Drop of blood (2-3 mm) in diameter: The blood can be obtained from finger Heal Microhematochrit tubes (non heparinised for EDTA or Heparinised for capillary blood) Wooden stick or glass capillary pipette Place either the long or the short edge of the pusher slide just in front of the blood drop, holding it at an approximately 30-degree angle from the patient slide. 8. The purpose of preparation of blood smear (blood film} is to study the morphology of RBCs, differential leukocyte count and reticulocyte count. Once blood has spread along the edge of the second slide then pull it away from the drop of blood firmly and swiftly. Puncture the ball of the finger, or in infants puncture the heel. Slide technique; Cover slip technique; Automated slide making and staining; What is the procedure of preparing peripheral smear ? The peripheral blood smear may have a blue hue and show rouleaux formation of the red blood cells because of a high level of immunoglobulin (Figure 21-3). Collect blood in an EDTA tube and make the smears when back at the clinic. To avoid this, I hold the pusher slide in my dominant hand with my thumb and index finger on the short edges and use the long edge to push the blood, thus keeping me from running off at an angle. Automated slide making and staining. 05 Nov 2020 by Goblusal. Clean the area to be punctured with 70% alcohol; allow to dry. Keep practicing with these guidelines in mind, and you will be making textbook-perfect slides in no time. It must be differentiated from the non-pathogenic microfilaria of Dipetalonema reconditum (“Dipet” for short). Second, let the blood spread all the way out before you start to push. It should end about two-thirds to three-fourths of the way down the slide. Peripheral blood film or Peripheral blood smear or Peripheral smear examination forms an important and basic lab investigation for diagnosing a number of hematological (blood) disorders. endobj
Agglutination of red blood cells and their decreased count in the blood smear, along with findings from t… 1) THIN BLOOD FILM Thin PBF can be prepared from anticoagulated blood obtained by venepuncutre or from free flowing finger prick blood by any of the following three techniques : 1. What is blood smear procedure.Sometimes a nurse may be required to take a blood smear specially when there is sudden rise of body temperature and a doctor is not available.. 7. Making a smear 1. Has this helped you? <>
Blood smear: slide-to-slide technique This item is available to registered subscribers only Sign up now to obtain ten tokens to view any ten Vetlexicon articles, images, sounds or videos, or Login Push the blood down the patient slide with the pusher slide. Techniques of making good bacterial smear. The end of the smear should be thin enough that it has a rainbow-like reflectiveness about it, and there should be no streaks at the very edge. 3. Pink to tan w/ violet/lilac granules. Tossed. Make the smear from fresh blood. <>
When making a wedge smear, there are two slides involved: the pusher slide and the patient slide. Select the finger to puncture, usually the middle or ring finger. 7 0 obj
You can also change the angle of the pusher slide. As with everything, practice makes perfect. Modified Knott’s Technique and Direct Blood Smear The modified Knott’s method is used for the concentration and identification of microfilariae, specifically the heartworm Dirofilaria immitis. The pusher slide pushes the blood down the patient slide to spread the sample out and create a monolayer of cells. When making a wedge smear, there are two slides involved: the pusher slide and the patient slide. The quality of the blood smear depends on a proper technique and quality of the staining. �9@�� v�.�
A high hematocrit level leads to thicker blood; a low hematocrit means thinner blood. Always start with room temperature, well-mixed, clot free, EDTA anticoagulated blood (heparin is used for some exotic species). <>
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To make your slide, there are just four steps to follow: With every slide you make, there are a couple of things that you should always do to ensure the best quality. endstream
If you have any other tips or insights on making peripheral blood smears, feel free to put them in the comments! q 16 0 0 1 0 0 cm/Image6 Do Q
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Allow the blood to spread along the edge of the slide. These are some of the errors I see almost every student make when they first start making slides. Blood smear analysis is a well known technique in medical laboratories. With the advent of hematological automated machines, complete and highly accurate hematological data and parameters like complete blood count or CBC can be obtained within a few minutes. Meeting these parameters gives you the best opportunity to see cells as they would be in vivo and approximately quantify them. 8 0 obj
A blood smear is a blood test used to look for abnormalities in blood cells. When done correctly, it should result in a uniform blood film, that gets progressively thinner. 2. Textbooks teach you to place the patient slide on the table and push the pusher slide, with the shorter edge touching the blood, away from you. Fixation of blood smear. stream
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Making blood smear slides can be critically important to hematologic investigation and diagnosis. You can control for this variation by adjusting the items listed above. Preparation of blood smear. When I make a slide, I have confidence that it will be a quality smear, which helps me maintain a consistent speed as I push. Name the types of peripheral blood smear. APPARATUS: 4-5 glass slides, compound microscope, pricking needle (blood lancet), spirit swab, cedar wood oil/liquid paraffin, Leishman’s stain, wash bottle, buffered water and staining tray. �I
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1 In private practice, rapid evaluation of blood smears alone can identify major hematologic changes, provided the specimens are well prepared and examined systematically. ... Making a combined thick and think smear for mammal blood is only possible if only one smear is made per slide. PDF | The process of medical diagnosis is an important stage in the study of human health. Left to right: • slide 1 – perfect smear • slide 2 – smear technique interrupted in middle • slide 3 – smear was skewed • slide 4 – blood droplet too thick • slide 5 – smear … Blood smear examination also is a useful quality control measure to verify leukocytosis, leukopenia, or thrombocytopenia, especially when blood count data apparently do not “fit” the clinical picture. Maintaining a consistent pushing speed prevents streaks on the edge of the slide as well as lines within the readable area. Hello, Friends... leishman stain procedure, ideal blood smear depends on a proper technique and of... 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